diff --git a/.Rhistory b/.Rhistory new file mode 100644 index 0000000..50ddc87 --- /dev/null +++ b/.Rhistory @@ -0,0 +1,512 @@ +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.breaks[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1,2)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.breaks[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1,2,3)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.breaks[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.breaks[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +#g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.breaks <- g.hist$breaks # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.breaks[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean)/kmin) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +alpha.LM +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean/kmin)) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1, lwd=5) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean/kmin)) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1, lwd=3) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean/kmin)) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1, lwd=3) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2, lwd=3) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +#plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3, lwd=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean/kmin)) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4, lwd=3) +plot(yeast) +hist(yeast) +hist(g.vec) +g.pois +g.mean +alpha.LM +alpha.ML +degree(g) +sort(degree(g)) +sort(degree(g),decreasing=FALSE) +sort(degree(g),decreasing=F) +sort(degree(g),decreasing=false) +sort(degree(g), decreasing = TRUE) +head(sort(degree(g), decreasing = TRUE)) +stddev(degree(g)) +sd(degree(g)) +tail(sort(degree(g), decreasing = TRUE)) +plot(log(g.breaks.clean), log(g.probs.clean)) +# Homework 4 for the University of Tulsa' s CS-7863 Network Theory Course +# Degree Distribution +# Professor: Dr. McKinney, Spring 2022 +# Noah Schrick - 1492657 +library(igraph) +library(igraphdata) +data(yeast) +g <- yeast +g.netname <- "Yeast" +################# Set up Work ################# +g.vec <- degree(g) +g.hist <- hist(g.vec, freq=FALSE, main=paste("Histogram of the", g.netname, +" Network")) +legend("topright", c("Guess", "Poisson", "Least-Squares Fit", +"Max Log-Likelihood"), lty=c(1,2,3,4), col=c("#40B0A6", +"#006CD1", "#E66100", "#D35FB7")) +g.mean <- mean(g.vec) +g.seq <- 0:max(g.vec) # x-axis +################# Guessing Alpha ################# +alpha.guess <- 1.5 +lines(g.seq, g.seq^(-alpha.guess), col="#40B0A6", lty=1, lwd=3) +################# Poisson ################# +g.pois <- dpois(g.seq, g.mean, log=F) +lines(g.seq, g.pois, col="#006CD1", lty=2, lwd=3) +################# Linear model: Least-Squares Fit ################# +g.breaks <- g.hist$breaks[-c(1)] # remove 0 +g.probs <- g.hist$density[-1] # make lengths match +# Need to clean up probabilities that are 0 +nz.probs.mask <- g.probs!=0 +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +plot(log(g.breaks.clean), log(g.probs.clean)) +g.fit <- lm(log(g.probs.clean)~log(g.breaks.clean)) +summary(g.fit) +alpha.LM <- coef(g.fit)[2] +lines(g.seq, g.seq^(-alpha.LM), col="#E66100", lty=3, lwd=3) +################# Max-Log-Likelihood ################# +n <- length(g.breaks.clean) +kmin <- g.breaks.clean[1] +alpha.ML <- 1 + n/sum(log(g.breaks.clean/kmin)) +alpha.ML +lines(g.seq, g.seq^(-alpha.ML), col="#D35FB7", lty=4, lwd=3) +plot(log(g.breaks.clean), log(g.probs.clean)) +g.breaks.clean <- g.breaks[nz.probs.mask] +g.probs.clean <- g.probs[nz.probs.mask] +plot(log(g.breaks.clean), log(g.probs.clean)) +## Set Working Directory to file directory - RStudio approach +setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) +#### Part A: Preparing Data +load("sense.filtered.cpm.Rdata") +# load phenotype (mdd/hc) data +subject.attrs <- read.csv("Demographic_symptom.csv", +stringsAsFactors = FALSE) +if (!require("dplyr")) install.packages("dplyr") +library(dplyr) +# grab intersecting X (subject ids) and Diag (Diagnosis) from columns +phenos.df <- subject.attrs %>% +filter(X %in% colnames(sense.filtered.cpm)) %>% +dplyr::select(X, Diag) +mddPheno <- as.factor(phenos.df$Diag) +# Normalized and transform +library(preprocessCore) +mddExprData_quantile <- normalize.quantiles(sense.filtered.cpm) +mddExprData_quantileLog2 <- log2(mddExprData_quantile) +# attach phenotype names and gene names to data +colnames(mddExprData_quantileLog2) <- mddPheno +rownames(mddExprData_quantileLog2) <- rownames(sense.filtered.cpm) +rownames(sense.filtered.cpm) +len(rownames(sense.filtered.cpm)) +length(rownames(sense.filtered.cpm)) +length(rownames(sense.filtered.cpm)) diff --git a/.~lock.Schrick-Noah_CS-6643_Lab-4.docx# b/.~lock.Schrick-Noah_CS-6643_Lab-4.docx# index 111e6d0..dc2814b 100644 --- a/.~lock.Schrick-Noah_CS-6643_Lab-4.docx# +++ b/.~lock.Schrick-Noah_CS-6643_Lab-4.docx# @@ -1 +1 @@ -,noah,NovaArchSys,29.09.2022 14:42,file:///home/noah/.config/libreoffice/4; \ No newline at end of file +,noah,NovaArchSys,30.09.2022 11:57,file:///home/noah/.config/libreoffice/4; \ No newline at end of file diff --git a/Schrick-Noah_CS-6643_Lab-4.R b/Schrick-Noah_CS-6643_Lab-4.R index bda527f..5038cf7 100644 --- a/Schrick-Noah_CS-6643_Lab-4.R +++ b/Schrick-Noah_CS-6643_Lab-4.R @@ -21,6 +21,7 @@ phenos.df <- subject.attrs %>% mddPheno <- as.factor(phenos.df$Diag) # Normalized and transform +if (!require("preprocessCore")) install.packages("preprocessCore") library(preprocessCore) mddExprData_quantile <- normalize.quantiles(sense.filtered.cpm) mddExprData_quantileLog2 <- log2(mddExprData_quantile) @@ -29,4 +30,54 @@ colnames(mddExprData_quantileLog2) <- mddPheno rownames(mddExprData_quantileLog2) <- rownames(sense.filtered.cpm) length(rownames(sense.filtered.cpm)) -#### Part B: Filter noise genes \ No newline at end of file + + +#### Part B: Filter noise genes +# coefficient of variation filter sd(x)/abs(mean(x)) +CoV_values <- apply(mddExprData_quantileLog2,1, + function(x) {sd(x)/abs(mean(x))}) +# smaller threshold, the higher the experimental effect relative to the +# measurement precision +sum(CoV_values<.045) +# there is one gene that has 0 variation -- remove +sd_values <- apply(mddExprData_quantileLog2,1, function(x) {sd(x)}) +rownames(mddExprData_quantileLog2)[sd_values==0] +# filter the data matrix +GxS.covfilter <- mddExprData_quantileLog2[CoV_values<.045 & sd_values>0,] +dim(GxS.covfilter) + + +#### Part C: Differential Expression with t-tests +# convert phenotype +pheno.factor <- as.factor(colnames(GxS.covfilter)) +pheno.factor +str(pheno.factor) +levels(pheno.factor) + +## Run t-tests +myrow <- 2 # first pick a gene row index to test +mygene<-rownames(GxS.covfilter)[myrow] +mygene + +# a. traditional R interface +mdd <- GxS.covfilter[myrow,pheno.factor=="MDD"] +hc <- GxS.covfilter[myrow,pheno.factor=="HC"] +t.result <- t.test(mdd,hc) +t.result + +# b. formula interface ~ saves a step +t.result <- t.test(GxS.covfilter[myrow,] ~ pheno.factor) +t.result +p <- t.result$p.value +t.result$statistic + +## Plot the Data +if (!require("ggplot2")) install.packages("ggplot2") +library(ggplot2) +# create data frame for gene +mygene.data.df <- data.frame(gene=GxS.covfilter[myrow,],phenotype=pheno.factor) +# boxplot +p <- ggplot(mygene.data.df, aes(x=phenotype, y=gene, fill=phenotype)) + + stat_boxplot(geom ='errorbar') + geom_boxplot() +p <- p + xlab("MDD versus HC") + ylab(mygene) +p diff --git a/Schrick-Noah_CS-6643_Lab-4.docx b/Schrick-Noah_CS-6643_Lab-4.docx index 3d88b9c..0c5c630 100644 Binary files a/Schrick-Noah_CS-6643_Lab-4.docx and b/Schrick-Noah_CS-6643_Lab-4.docx differ